Biorbyt's CD45RA Antibody (Leukocyte marker) is a Mouse monoclonal antibody. The CD45RA Antibody (Leukocyte marker) was generated using PTPRC as the antigen. It reacts with Homo Sapiens, and Human. This antibody has been shown to work in applications such as: Cell Sorting, Isolation, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot.
Description
CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve)